Technician II

The Lunenfeld-Tanenbaum Research Institute of Mount Sinai Hospital, a University of Toronto affiliated research centre, is one of the world’s leading centres in biomedical research. With ground-breaking discoveries in research areas such as diabetes, genetic disorders, cancer and women’s and infants’ health, the Institute is committed to excellence in health research and the training of young investigators. Strong partnerships with the clinical programs of Mount Sinai Hospital ensure that scientific knowledge is used to promote human health. Your significant contributions will assist in maintaining our momentum in advancing our research.

The Jackson lab is focused on the use of multiplexed imaging and single cell analyses to understand the formation and function of multi-cellular tissue subunits, communities of cells. Using imaging mass cytometry combined with multiple other technologies we track and investigate changing cellular interactions within pre-clinical models of disease and clinical patient samples. We are looking for a collaborative and detail oriented Technician to support varied cancer research and methods development projects in the lab while helping to coordinate the management of the lab.

POSITION OVERVIEW:

The Technician / Lab Coordinator will be responsible for contributing to our imaging mass cytometry experimental pipeline as well as support of day-to-day lab operations and contribute to diverse research projects.

DUTIES AND RESPONSIBILITIES:

• Acquire bright field, fluorescence and high dimension images using microscopy and CyTOF Suspension and Imaging Systems
• Help manage tissue sample, antibody, cell line and plasmid inventories
• Assist with mouse genotyping, breeding program, and surgical procedures
• Dissect, fix, section, and process tissue
• Maintain animal user protocols
• Assist with tissue sample, antibody, cell line, and plasmid inventory management
• Establish SOP for diverse experimental protocols
• Administer lab safety programs to ensure compliance
• Onboard and train new lab members
• Help with ordering of lab supplies
• Coordinate usage and maintenance of shared lab equipment and reagents
• Organize shared lab duties for the efficient operation of the lab
• Collaborate with other lab members to execute experimental work

SKILLS/QUALIFICATIONS:

• Minimum BSc in biological sciences. Additional work experience preferred
• Experience in the following techniques is an asset:
  • Molecular biology (cloning, PCR, conjugation biochemistry)
  • Immunohistochemistry and tissue processing (fixation, antigen retrieval, immunostaining, imaging)
  • Microscopy
  • Flow cytometry or other single cell and antibody based measurements
  • Mammalian cell culture (transfections, lentiviral infection, tissue culture)
  • Animal husbandry
  • Statistics and data analysis using scripts and code
• Excellent interpersonal, communication, organizational, and recordkeeping skills
• Ability and eagerness to learn new techniques
• Ability to work independently and in a team environment

In accordance with Institute’s policy and legislated health and safety requirements, employment is conditional upon the verification of credentials, completion of a health review, and demonstrating proof of immunity and vaccination status of vaccine-preventable diseases. All employees and affiliates will follow safe work practices and comply with health and safety policies, procedures, and training. Successful candidates will be required to provide two (2) written reference letters from their former employer(s)/supervisor(s).

To ensure the safety and wellbeing of all our people and patients, Sinai Health has made it a priority to support everyone in getting vaccinated against COVID-19. Consistent with this, our Staff Immunization & Surveillance Policy was recently updated to include the requirement for full vaccination – currently defined as receiving two doses and serving a 14-day waiting period following the second dose – in order to be able to work at Sinai Health. To be compliant with our updated Policy, you must provide proof of dual COVID-19 vaccination, as indicated on the Prospective Immunization and Surveillance Policy Information Sheet, in order to be eligible for employment at Sinai Health. If you believe you are one of the very few people who may require an exemption from vaccination, supporting medical information must be submitted to our Occupational Health department, who will review and assess.

We are a fully committed to fairness and equity in employment and our recruitment and selection practices. We encourage applications from Indigenous peoples, people with disabilities, members of sexual minority groups, members of racialized groups, women and any others who may contribute to the further diversification of our Sinai Health community. Accommodation will be provided in all parts of the hiring process as required under our Access for People with Disabilities policy. Applicants need to make their requirements known in advance.

The Lunenfeld-Tanenbaum Research Institute is a scent sensitive environment, and all members of the community are expected to refrain from wearing or using scented products while visiting or working at the Institute. We also support a barrier-free workplace supported by the Institute’s accessibility plan, accommodation and disability management policies and procedures. Should you require accommodation at any point during the recruitment process, including accessible job postings, please contact the Lunenfeld-Tanenbaum Human Resources Department.

Hours:37.5 hours per week

Contact Name:Online
Contact Email:Online
Contact Phone:Online